RNAseq quantitative analysis of ion channel mRNA expression in microbead occlusion model
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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资源简介：
The purpose of this study was to utilize RNAseq to determine if there are changes in ion channel mRNA expression in the retina of C57BL/6 mice subjected to the Microbead Occlusion Model. Whole retina was isolated from 2 month old mice that received intracameral injections of either saline or 15-um-diameter microbeads. Total RNA was isolated from the retinas and subjected to RNA sequencing using Illumina HiSeq 2500. Fold change in mRNA expression was compared between saline- and microbead-injected samples. We then analyzed these data for changes in expression of potassium channels, such as tandem-pore domain K+ channels, inwardly rectifying K+ channels, and the Na+/K+-ATPase.
提供机构：
Vanderbilt University Medical Center
创建时间：
2022-02-20
