Transcriptome analysis of soybean roots in response to boron deficiency

Boron (B) is an essential micronutrient essential for normal plant normal growth and development. B deficiency is detrimental to Glycine max (L.) Merr., which is one of the leading oil-crops. species, is extremely susceptible to B deficiency. In this study, the physiological analysis of soybean seedlings under B deficiency (T1, T2, T3, T4) and control (CK1, CK2, CK3, CK4) after 12 h, 24 h, 72 h, and 8 d was carried out, and the roots were subjected to transcriptome sequencing analysis. The results showed that under B deficiency, the plant height, SPAD, and chlorophyll fluorescence value (Fv/Fm) of soybean seedlings decreased significantly, indicating that B stresses significantly inhibited plant growth and photosynthesis. After RNA-seq sequencing, showed a total of 5126 DEGs (Differentially Expressed Genes) were obtained, including 953 DEGs at 12 h, 760 DEGs at 24 h, 1945 DEGs at 72 h and 1468 DEGs at 8 d. There were 9Nine DEGs co-existed at the four-time treatmentspoints, among which GLYMA_03G130600 regulated regulates transcription factor ORG3, which was is related to plant growth. GO analysis showed thatrevealed a total of annotated 8763 DEGs were annotated, among which the terms plasma membrane, extracellular, enzyme activity, ion transport metabolic process and oxidoreductase activity were significantly enriched at the four -time treatmentspoints, which might hinder the growth of soybean and accelerate senescence. KEGG analysis showed that at level 1, the DEGs were mainly enriched in photosynthesis-related, phenylpropanoid biosynthesis, nitrogen metabolism, and plant hormone signal transduction. At level 2 of secondary metabolism, the DEGs were mainly enriched in energy metabolism and amino acid metabolism pathways, which indicates that B stresses mainly affect photosynthesis, hormone regulation and amino acid metabolism of soybean, thus affecting plant growth. Through The above results, give deeper more comprehensive insights into the soybean response to B deficiency would be focused and lay a the foundation for the further studies of the molecular mechanism of soybean response to boron deficiency.