Nuclear inclusion X testing of Pacific Razor clams from select locations in Washington and Alaska

A quantitative PCR (qPCR) assay was developed to detect nuclear inclusion X (NIX) in Pacific razor clams, and assay specificity was confirmed by chromogenic in situ hybridization (CISH). Both tests were applied to evaluate NIX infections in wild Pacific razor clams collected during spring 2019. Consistent with results from earlier histopathological assessments, qPCR and CISH indicated 100% prevalence in razor clams from two Washington beaches and 0% prevalence from two Alaskan beaches.