Expression data from retinoic acid-sufficient and retinoic acid-deficient human airway smooth muscle [human primary ASMs]

Observational studies in human suggest involvement of vitamin A/retinoic acid (RA) signaling in the regulation of airway smooth muscle (ASM) function, but the precise mechanisms by which RA impacts ASM phenotype is not clear. Here, we generated trascriptional profiles from primary human ASM from 3 unrelated donoros cultured in control medium or medium containing BMS493 (an retinoic acid receptor antagonist) Human primary ASMs from 3 unrelated donors were cultured in control medium, BMS-containing medium (1 micromolar) and DEAB-containing medium (10 micromolar) for 24 hours prior to the extraction of RNA for transcriptional profile analysis.