Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development - in vivo imaging of OV formation

Development of a functional retina is accompanied by several morphological events transitions that ultimately result in the formation of a functional retina. One of them is the formation of the OV. When fish-derived organoids were generated with reduced cell-seeding density by 50%, the organoids formed individual domains reminiscent of the forming optic vesicle in vivo. To address the mode of OV formation in fish organoids, we employed live imaging of Rx3::H2B-GFP-derived organoids from day 1 to day 2 of development. This dataset is a raw data from live imaging of Rx3::H2B-GFP-derived organoids from day 1 to day 2 of development performed on the 16× detection MuVi SPIM Multiview light-sheet microscope (Luxendo Light-sheet, Bruker Corporation).