EFNB1 is essential for modulating the migratory capacity of ERMS cells.

Summary of (A) scratch assays and (B) transwell migration assays on RD and 381T cells with EFNB1 knockdown by two independent siRNAs. Summary of (C) scratch assays and (D) transwell migration assays performed on control GFP-overexpressing and EFNB1-overexpressing RD cell lines. (E) ChIP assays showing differential binding of acetyl-histone H3 (Lys9) on EFNB1 promoter in RD cells treated with DMSO, 200 nM TSA, or 1μM SAHA. Rabbit IgG was used as a negative control for chromatin immunoprecipitation. (F) Western blot assessing EFNB1 protein expression level in RD cells treated with DMSO, 200 nM TSA or 1 μM SAHA. Percent intensity relative to DMSO (vehicle) treatment is shown. GAPDH was used as a loading control.