Gene expression profiling of developing murine Müller glia

To study molecular changes during differentiation of retinal progenitor cells (RPCs) into Müller glia, we isolated Notch1+ cells (Notch1 is a marker of RPCs) from postnatal-day (P) 0, 3, 7, and 14 retinas,as well as Glast + cells (Glast/Slc1a3 is a marker of Müller glia precursors and Müller glia in the retina) from P7, P14, P21, and P28 retinas. We studied gene expression in these cells using MEEBO microarrays. To characterize and compare P0, P3, P7, and P14 Notch1+ cells (RPCs) as well as P7, P14, P21, and P28 Glast+ cells (Müller glia precursors and adult Müller glia), RNA extracted from these cells was used for microarray analysis. A total of two-three independent biological replicates at each time point were obtained for comparative profiling of purified cells.