Raw diffraction images of heliorhodopsin
收藏资源简介：
Crystal structure of heliorhodopsin from <em>Thermoplasmatales archaeon </em>(PDB code: 6IS6). Small-wedge (10-15°/crystal) datasets collected from loop-harvested microcrystals using EIGER X 9M detector at a wavelength of 1 Å on BL32XU, SPring-8. Beam size was around 15×10 µm<sup>2</sup> and oscillation step was 0.1°. The crystals belonged to space group C222<sub>1</sub> with unit cell parameters a~52, b~110, c~108 Å. In total 212 small-wedge and two helical (180 & 120°) datasets were collected, and of these 179 datasets were indexed and integrated using XDS with KAMO pipeline. Finally 107 integrated results were merged at 2.4 Å resolution after CC-based clustering and outlier rejection by KAMO in the published result (Shihoya et al. Nature, 2019). This upload also includes SeMet-labeled heliorhodopsin data (not actually used for phasing) and a different crystal form (tetragonal). SeMet data could be merged in P2<sub>1</sub>2<sub>1</sub>2<sub>1</sub> space group at 3.8 Å resolution. The tetragonal data are at 2.8 Å resolution.
