16S rRNA gene sequencing of the intestinal microbiota in Pacific white shrimp (Litopenaeus vannamei) fed with dietary cannabidiol (CBD)

Intensive aquaculture practices frequently subject the Pacific white shrimp (Litopenaeus vannamei) to environmental stressors, which can compromise intestinal health and increase susceptibility to opportunistic pathogens. Cannabidiol (CBD) is a promising bioactive phytocannabinoid known for its anti-inflammatory and stress-mitigating properties, but its modulatory effect on the aquatic gut microecosystem remains unexplored. This project contains the high-throughput 16S rRNA gene amplicon sequencing data of the intestinal microbiota from juvenile L. vannamei supplemented with varying levels of dietary CBD. In this study, shrimp were fed a basal diet supplemented with CBD at concentrations of 0 (control), 10, 20, 40, and 80 mg/kg for an 8-week feeding trial. At the end of the trial, the intestinal contents were aseptically collected for total genomic DNA extraction. The hypervariable V3-V4 region of the bacterial 16S rRNA gene was amplified and sequenced on the Illumina NovaSeq 6000 platform. The primary objective of this sequencing project is to evaluate the dose-dependent impacts of dietary CBD on the intestinal microecology of L. vannamei, specifically focusing on structural optimization, microbial diversity shifts, and the potential suppression of aquaculture pathogens. These data provide fundamental insights into the host-microbiome interactions and the application of CBD as a functional feed additive in crustacean aquaculture