The CTD of EBF1 enhances Brg1 recruitment to chromatin

EBF1 binds lymphoid progenitor chromatin prior to the detection of chromatin accessibility, whereby a C-terminal domain (CTD) enables “pioneering” independently of cooperating transcription factors. The CTD of EBF1 includes a short prion-like domain (PLD) with an ability of liquid-liquid phase separation in vitro and in vivo. The PLD of EBF1 mediates the recruitment of the chromatin remodeler Brg1 (a subunit of SWI/SNF remodeling complex). 5 anti-Brg1 ChIP-seq samples in fetal liver EBF1 -/- c-kit+ lymphocyte progenitors transduced with different vectors (empty vector and different EBF1 versions wt, dC, d480-520, F-GATA3) and 1 input control. 2 anti-Brg1 ChIP-seq samples in Abelson murine leukemia virus (A-MuLV) transformed pro-B cells with EBF1 replacemnt (by EBF1 wt or dC) and 2 input controls. 2 anti-EBF1 ChIP-seq samples in Abelson murine leukemia virus (A-MuLV) transformed pro-B cells with EBF1 replacemnt (by EBF1 wt or dC) and 2 input controls.