m6A epitranscriptome regulates axonogenesis and reprogramming of retinal ganglion cells

induced retinal ganglion cells (iRGCs) is an excellent system to generate RGC-like neurons for translational and theoretical studies. N6-methyladenosine (m6A) is the most prevalent internal modification in mRNAs. Using this iRGC system, we demonstrated that Mettl3, the core component of the m6A-methyltransferase complex, promotes iRGC axon development and the final iRGC reprogramming outcome. Overall design: mouse embryonic fibroblasts (MEFs) infected with ABI-2A-GFP lentiviruses were collected at day 5 after induction (DAI 5) and DAI 13 , and processed for MeRIP-Seq.