Statistical significance test results for choice of binning method on ASTRAL.

We performed ANOVA to test the significance of the choice of methods (unbinned, weighted binned, unweighted binned, WSB-50: weighted statistical binning using 50% bootstrap support threshold and WSB-75: weighted binning using 75% bootstrap support threshold). For weighted vs. unweighted, we compared 50% bootstrap support threshold for avian, 75% for mammalian, and both 50% and 75% for 15- and 10-taxon datasets. All p-values are corrected for multiple hypothesis testing using the FDR correction (n = 14). “n.a.” stands for “not available”.