scRNA-seq of myeloid cells from subcutaneous MC38-Ova tumours and draining lymph nodes (LN) in photoconvertible Kaede mice

We combined scRNA-seq and a photoconvertible mouse model to study the spatiotemporal dynamics of macrophages and dendritic cells (DCs) in murine tumours including their migration to the lymph node, including the effects of immune checkpoint therapy (anti-PD-L1). Overall design: Myeloid cells in tumours were isolated by fluorescence-activated cell sort (FACS) using LiveCD45+TER119-Kaede+CD11b+NK1.1- signal, followed by separation of Kaede-green or Kaede-red fractions for analysis with scRNA-seq. Kaede-red immune cells in the tumour-dLN (representing cells that have migrated from the tumour) were isolated using LiveCD45+TER119-Kaede-red+ signal.