Empirical Validation of Pooled Reqsequencing

Sequencing of pooled, non-barcoded individuals holds great promise for the inexpensive and efficient assessment of genome-wide population allele frequencies. This approach has yet to be tested with whole, complex eukaryotic genomes. In order to carry out such a test, we sequenced a series of libraries made from pools of largely isogenic, individually sequenced Drosophila melanogaster strains. We compared allele frequency estimates derived from these "pooled" libraries to corresponding estimates derived from individually sequenced strains.