Single cell RNA sequencing of placenta in patients with gestational diabetes during term pregnancy

We performed the single cell RNAsequencing in the placental tissues of term pregnancies with gestational diabetes mellitus complicated with macrosomia after cesarean section to explore the transcriptome differences and potential mechanisms of placenta in the process of macrosomia in patients with gestational diabetes. Our research helps to understand placental overgrowth in GDMM and its impact on fetal development, providing new insights into the pathogenesis of GDMM and predicting potential therapeutic interventions. Overall design: After collecting placental tissues, they were immediately processed into single-cell suspensions. The process involved washing, cutting, digesting, filtering, lysing red blood cells, removing dead cells, staining, and finally suspending the cells in a solution. The single-cell suspension was loaded onto the Chromium Controller to generate single-cell gel beads, followed by single-cell RNA sequencing using the Illumina Novaseq6000 sequencer.