Comparison of infloresence transcriptome of bp er vs. bp er fil. Arabidopsis thaliana

BP and ER encode proteins that act synergistically to regulate Arabidopsis inflorescence architecture. To search for genes/proteins that influence the BP/ER signaling pathways, we conducted mutagenesis of the bp er double mutant and found that a mutation in FILAMENTOUS FLOWER (FIL) suppresses many of the morphological/developmental defects in bp er. Given that FIL encodes a Zn-finger containing transcription factor, microarray analysis was conducted on bp er vs. the bp er fil line to identify genes that are misregulated and which might implicate specific genes/proteins/pathways that are involved in regulating inflorescence development. Overall design: Plants of the bp er parent line and the bp er fil suppressor line were grown in Conviron growth chambers. Inflorescences that contained no more than 2 stage 13 flowers were harvested and triplicate samples were ultimately compared.