The impact of various lengths of inverted sequence on NDE1 gene deletion efficiency.
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*The success rate was based on the PCR analysis of 10 colonies randomly selected from the selective plates. The correct initial integration of the disruption gene cassette was analyzed by PCR amplification with the confirmation oligos C1 and C2, and the excision rate was based on PCRs using the confirmation oligos C1 and C4.
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2015-12-02



