Acceleration of slow refolding phase λF3(RS) by E. coli trigger factor.
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The slow refolding phase λF3(RS) of 0.5 µM CMPK was analyzed at 360 nm during refolding in 0.6 M urea at different concentrations (0–5 µM) of E. coli trigger factor (TF). The ratio of λF3(RS)(TF) to the refolding rate constant without trigger factor λF3(RS)(TF = 0) is displayed as a function of TF concentration. Increasing concentrations of TF lead to an acceleration of λF3(RS). At a ratio of 2∶1 (1 µM TF) the ratio reaches a maximum of 1.6.
创建时间:
2016-02-23



