Detection of Neoplasms by Metagenomic Sequencing of Cerebrospinal Fluid

Detection of Neoplasms by Metagenomic Sequencing of Cerebrospinal Fluid

Wei Gu, MD, PhD1,2*, Andreas M. Rauschecker, MD, PhD3, Elaine Hsu, BS1, Kelsey C. Zorn, BS4, Yasemin Sucu, BS1, Scot Federman, BS1, Allan Gopez, BS1, Shaun Arevalo, BS1, Hannah A. Sample, BS4, Eric Talevich, PhD5, Eric D. Nguyen, MD, PhD 1, Marc Gottschall, BS1, Bardia Nourbakhsh, MD, MAS6, Carl A. Gold, MD, MS7, Bruce A.C. Cree, MD, PhD, MAS8, Vanja Douglas, MD8, Megan B. Richie, MD8, Maulik P. Shah, MD, MHS8, S. Andrew Josephson, MD8, Jeffrey M. Gelfand, MD, MAS8, Steve Miller, MD, PhD 1, Linlin Wang, MD1, Tarik Tihan, MD, PhD9, Joseph L. DeRisi, PhD4,10, Charles Y. Chiu, MD, PhD11,12, Michael R. Wilson, MD, MAS8*

1Department of Laboratory Medicine, University of California San Francisco, CA 94107, USA

2Department of Pathology, Stanford University, CA 94305, USA

3Department of Radiology and Biomedical Imaging, University of California San Francisco, CA 94107, USA

4Department of Biochemistry and Biophysics, University of California San Francisco, CA 94107, USA

5DNANexus, Mountain View, CA 94040, USA

6Department of Neurology, Johns Hopkins University, MD 21287, USA

7Department of Neurology, Stanford University, CA 94305, USA

8Weill Institute for Neurosciences, Department of Neurology, University of California San Francisco, CA 94107, USA

9Department of Pathology, University of California San Francisco, CA 94107, USA

10Chan Zuckerberg Biohub, San Francisco, CA 94107, USA

11UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA 91407, USA

12Department of Medicine, Division of Infectious Diseases, University of California San Francisco, CA 94107, USA

* Corresponding authors:

Michael Wilson, MD, MAS

UCSF Department of Neurology

675 Nelson Rising Lane, NS212

San Francisco, CA 94158

[e-mail]

Wei Gu, MD, PhD

Stanford University, Department of Pathology

3373 Hillview Ave, 218

Palo Alto, CA 94304

650-723-1914

[e-mail]