Temporal transcriptome analysis of Bacillus subtilis NDmed in the submerged biofilm model

Investigation of the kinetics of whole genome gene expression level changes in Bacillus subtilis NDmed strain during formation of submerged biofilm and pellicle. The Bacillus subtilis NDmed strain analyzed in this study is able to form thick and highly structured submerged biofilms as described in Bridier et al., (2011) The Spatial Architecture of Bacillus subtilis Biofilms Deciphered Using a Surface-Associated Model and In Situ Imaging. PLoS ONE 6(1):e16177. A seven chip study using total RNA recovered from static cultures of Bacillus subtilis NDmed growing in TSB medium in 96-well microplates for different times after adhesion to the bottom of the wells. Each chip measures the expression level of 5,737 transcripts from Bacillus subtilis 168.