Hodel et al Original Western blot data

Original Western blot data used for Figure S3 in Hodel et al. 50 ug of cell lysate from the indicated cell lines were separated via SDS-PAGE and transferred to nitrocellulose membrane using standard conditions. The blot was then physically cut into sections and stained with the indicated primary antibodies, inlcuding p53, P-p53, Mdm2, p21, PUMA and GAPDH as a loading control. Longer exposures are also provided for Mdm2 and p53.

Hodel 等人所著文献中图 S3 所使用的原始 Western blot 数据。经分离胶电泳（SDS-PAGE）分离的指定细胞系裂解物 50 微克，在标准条件下转移至硝酸纤维素膜上。随后，Western blot 被物理切割成多个部分，并使用指定的主要抗体进行染色，包括 p53、P-p53、Mdm2、p21、PUMA 以及 GAPDH 作为加载对照。此外，还提供了 Mdm2 和 p53 的较长曝光时间图像。