Different expression of circRNAs in blood from idiopathic short stature patients and healthy controls

Idiopathic short stature is diagnosed by a standing height of less than two standard deviation scores in a specific population adjusted for age and gender and the exclusion of identifiable diseases. A series of studies have confirmed that noncoding RNAs can regulate the chondrocyte proliferation, hypertrophy, and endochondral ossification in the growth plate. In order to analyze and find differentially expressed circRNAs in Idiopathic short stature and healthy controls, we aimed to explore whether differentially expressed circRNAs in idiopathic short stature. Four pairs of blood samples were subjected to microarray analysis using the Arraystar Human CircRNAs Microarray v2 (Arraystar, USA). Compared to normal individuals, in ISS patients, the expression levels of 83 circRNAs were upregulated and those of 62 were downregulated. Four pairs of blood specimens from idiopathic short stature patients and healthy controls were used for circRNAs microarray analysis. RNA samples were prepared for microarray hybridization using Eastep Super RNA simple Total RNA Kit according to the manufacturer’s protocol. Total RNA was digested using RNase R to enrich the sample for circRNAs and eliminate linear RNAs.