Research data in support of "Haptotactic motion of multivalent vesicles along ligand-density gradients"

OVERVIEW OF EXPERIMENTAL TIMELAPSE/DNA COVERAGE DATA Experimental micrographs are organised into .zip files corresponding to sticky end length. For each sticky end length (l=5,6,7), we have included micrographs from a single experimental run as a representative example. The micrographs were collected with a Nikon Eclipse Ti2-E inverted microscope using a Nikon CFI Plan Apochromat Lambda D 10x dry objective (NA 0.45). For each experimental run, there are two types of files: 1) Timelapse recording of GUVs. These have been split in multiple files due to size limitations on the repository. 2) Image of DNA coverage on the substrate surface The experimental file format is .tif, which can be read using ImageJ (https://imagej.net/ij/download.html). For the time lapse data, each .tif file represents a time-series of images taken over a distinct region of the substrate (bottom of a well in a 96-well plate). The images of the DNA coverage on these regions of the substrate are saved in separate .tif files, using the same series numbering. Note that the time lapse files are split into two or three .zip files per sticky end length, due to limitations on the size of files uploaded to the repository. The corresponding metadata are saved in .txt files. --- OVERVIEW OF SIMULATION DATA Simulated trajectory data are organised into .zip files corresponding to the sticky end length (l=5, 6 and 7; the data for each sticky end length are additionally grouped by vesicle radius (R=100nm, 500nm and 900nm). For each combination of sticky end length and vesicle radius, there are two sets of data: 1) Mean vesicle displacement (and standard error of the mean displacements) over time. Mean displacements parallel and perpendicular to the gradient are in files with names starting "X_" And "Y_", respectively. Standard error of the mean displacements are in files with names starting "dX_" And "dY_". The corresponding timepoints are stored in files with names starting "t_". 2) The final/total displacements of each individual vesicle are stored in files with names starting with "X_end_" and "Y_end_". The simulation data file format is .npy, which can be read using the NumPy library in Python --- FURTHER DETAILS Please see the main manuscript and SI for further details on samples and data collection methods.