CIL:39968, Mus musculus, CNS neuron (sensu Vertebrata). In Cell Image Library
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Individual cells in fixed tissue were injected with Lucifer yellow and stored in 4% paraformaldehyde at 4° C. Fluorescence was photoconverted using DAB and a confocal laser light source. After this process, tissue was washed thoroughly in PBS and processed for EM by osmication, dehydration, and embedding
in epoxy resin. For detailed methods, see the protocol provided in the CCDB. The tomogram was generated using an Hitachi UHVEM. Single tilt images spanned -66 to 66° in 2° increments.
提供机构：
UC San Diego Library Digital Collections
创建时间：
2021-06-17
