Results of the computational and experimental phases for the candidate proteins, positive and negative controls.

PredHel: number of transmembrane helices predicted by TMHMM 2.0; (+): positive control proteins, (−): negative control proteins; PA-SUB v.2.5 predictions are classified as: NA: Not applicable. C: cytoplasmic; E: extracellular; PM: plasma membrane. In Phobius predictions: TM indicates the number of predicted transmembrane segments, SP: indicates if there is a predicted signal peptide. TPS: Protein recognition by the specific immune sera in M. tuberculosis H37Rv total protein sonicate; SF: Protein recognition by the specific immune sera in M. tuberculosis H37Rv membrane and culture filtrate (for positive controls and candidate proteins) or cytoplasm (for negative controls) by subcellular fractionation. IEM: Protein recognition on the surface of intact M. tuberculosis H37Rv bacillus by immune electron microscopy. None of these proteins was part of the training data sets of any of the Machine Learning tools.