CircRNAs full-length sequencing in the human and mouse brain samples using Oxford Nanopore Technology

We optimized a protocol to enrich, digest and add poly(A) tail to the circular RNAs in order to make them compatible with the Oxford Nanopore Technology for full-length sequencing Circular RNAs from human cortex and total mouse brain was sequenced我们优化了一套实验方案，用于富集、酶解环状RNA（circular RNAs）并为其添加多聚腺苷酸尾（poly(A) tail），使其适配牛津纳米孔测序技术（Oxford Nanopore Technology）以开展全长测序。本研究对取自人类大脑皮层与小鼠全脑的环状RNA进行了测序。