ChIP-seq analysis of STAT1-WT and STAT1-K193R gastric cancer cells

This dataset presents ChIP-seq profiles of STAT1 wild-type (STAT1-WT) and lactylation-deficient mutant (STAT1-K193R) in MGC803 gastric cancer cells. The study aims to investigate the impact of STAT1-K193 lactylation on chromatin binding patterns. Comparative analysis of STAT1 occupancy between the two cell lines provides insights into the regulatory roles of STAT1 post-translational modification in gastric cancer progression. Chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) was performed in MGC803 cells stably expressing either STAT1-WT or STAT1-K193R. Cells were cross-linked, sonicated, and subjected to immunoprecipitation using anti-STAT1 antibody. Input DNA and IP samples were sequenced using the Illumina platform. Each condition was processed in biological replicates to ensure reproducibility.