Mre11 physically interacts with Rad18 in vivo.
收藏Figshare2016-02-23 更新2026-04-29 收录
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https://figshare.com/articles/dataset/_Mre11_physically_interacts_with_Rad18_in_vivo_/1218069
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Asynchronous W303 tagged yeast strains containing either Rad18-HA and Mre11-Myc, Rad18-HA alone, or Mre11-Myc alone were used for analysis in this experiment. Strains containing only Rad18-HA or only Mre11-Myc tags were used as negative controls. Cells were grown to 1×107 cells/ml before being treated with or without 0.05% MMS (as indicated) for 90 minutes. Cells were then cross-linked with formaldehyde prior to cell lysis and the lysates were immunoprecipitated with either (A) anti-HA antibody, or (B) anti-Myc antibody. Lysates were also incubated with uncoupled beads as another negative control as shown in A and B. Whole cell extracts and immunoprecipitates were then analyzed by western blot analysis with anti-Myc and anti-HA antibodies.
创建时间：
2016-02-23
