ENCSR636HFF

exclusion list - The DAC Exclusion List Regions (previously named "DAC Blacklisted Regions") aim to identify a comprehensive set of regions in the human genome that have anomalous, unstructured, high signal/read counts in next gen sequencing experiments independent of cell line and type of experiment. There were 80 open chromatin tracks (DNase and FAIRE datasets) and 20 ChIP-seq input/control tracks spanning ~60 human tissue types/cell lines in total used to identify these regions with signal artifacts. These regions tend to have a very high ratio of multi-mapping to unique mapping reads and high variance in mappability. Some of these regions overlap pathological repeat elements such as satellite, centromeric and telomeric repeats. However, simple mappability based filters do not account for most of these regions. Hence, it is recommended to use this exclusion list alongside mappability filters. The DAC Exclusion List Regions track was generated for the ENCODE project. - ENCODE - U01HG004695 - Ewan Birney, EBI

排除列表 - DAC 排除列表区域（之前命名为“DAC 黑名单区域”）旨在识别人类基因组中一系列具有异常、非结构化和高信号/读数计数的区域，这些区域在下一代测序实验中独立于细胞系和实验类型。共使用了80条开放染色质轨迹（DNase和FAIRE数据集）和20条ChIP-seq输入/控制轨迹，覆盖约60种人类组织类型/细胞系，以确定这些具有信号伪迹的区域。这些区域往往具有非常高的多映射到唯一映射读数的比率以及可映射性的高变异性。其中一些区域与病理性的重复元素（如卫星、着丝粒和端粒重复）重叠。然而，基于简单可映射性的过滤器无法解释这些区域中的大多数。因此，建议在可映射性过滤器的基础上使用此排除列表。DAC排除列表区域轨迹是为ENCODE项目生成的。ENCODE - U01HG004695 - Ewan Birney, EBI