Effect of SRSF1 depletion on FANCD2 mRNA and isoform expression and effect of FANCD2 depletion or expression of SRSF1 mutants on splicing. Effect of SRSF1 depletion on FANCD2 mRNA and isoform expression and effect of FANCD2 depletion or expression of SRSF1 mutants on splicing

To investigate the cooperative function of FANCD2/SRSF1 complex in the regulation of R-loops, we performed gene expression, isoform and splicing analysis in Hela cells depleted of SRSF1 or expressing SRSF1 mutants and FA-D2 mutant (FA-D2) and wild type (FA-D2+FANCD2) cells. Overall design: FANCD2 gene expression and isoform analysis of RNA-seq data for HeLa cells depleted for SRSF1. Differential splicing analysis of RNA-seq data for HeLa cells expressing SRSF1 mutants or FA-D2 mutant (FA-D2) and wild type (FA-D2+FANCD2) cells.