MAGE-seq E. coli primary sigma factor RpoD amplicon high-throughput genotyping. MAGE-seq E. coli primary sigma factor RpoD amplicon high-throughput genotyping

Genotyping of RpoD mutants via amplicon sequencing from the following manuscript: \"Systematic dissection of σ70 sequence diversity and function in bacteria\" by Park and Wang (2020). Includes raw sequencing reads from samples from MAGE-seq single codon saturation mutagenesis and high-throughput fitness competition experiment as well as the RpoD ortholog mutants generated through recombineering and CRISPR selection.