森林中食藥用菇類的開發利用及種源保存(3/3) Utilization and cultural preservation of edible and medicine mushrooms from forests（3/3）

將供試5種段木進行高溫滅菌後，植入牛樟菌種，在植菌後1-2個月，供試5種木材均可觀察到白色點狀菌絲生長，6個月後二葉松與牛樟木材上之牛樟菇菌絲已逐漸轉變紅色，但仍未有出菇。分析6種樹木木材(牛樟、樟樹、冇樟、杉木、香杉及柳杉)上產生牛樟菇菇體成分差異，將菇體以95%酒精萃取，將酒精萃取液以HPLC分析，進行牛樟菇8種 指標成分比較，結果顯示，6種木材產生之牛樟菇菇體材料，均可測出選
用之八種成分( a n t c i n A 、B 、C 、H 、K 、d e h y d r o s u l p h u r e n i c a c i d 、dehydroeburicoic acid及4,7-dimethoxy-5-methyl-1,3-benzodioxole)，此結果說明不同木材基質形成之菇體，其主要八種指標成分均會出現。在中南部共5次菇菌
野外調查採集中，共獲得75個大型真菌標本，並分離到30種菌種。其中有3株相鄰小孔菌、3株小孔硬孔菌、2株南 方靈芝、3株靈芝、5株熱帶靈芝及6株桑黃類與9株未鑑定的無摺菌菌株。
The aims of this study are (1) to product the fruiting body of Antrodia cinnamomeaon different wood substrates and analyze different of
indicatingcomponents among different wood substrates. (2) To collect culture and preserve edible and medicinalmushrooms such as tuber，Cordyceps spp、Antrodia cinnamomea and macrofungi﹙ in forests. Five selected wood (Chamaecyparis formosensis 、C.obtusa var formosana Cinnamomum kanehirae 、Cryptomeria japonica and Pinus taiwanensis )were autoclaved before A.cinnamomea was inoculated. The inoculated woods were observed the white spots of growth mycelica1-2 month after inoculation .
The inoculation woods of Ci.kanehirae and P.taiwanesis turned reddish 6 months after inoculation, but no showing fruiting body formation
yet.Eightindicating components in A.cinnamomea inclndingantcin A、B、C、H、 K、dehydrosulphurenic acid、dehydroeburicoic acid and 4,7-dimethoxy-5-methyl- 1,3-benzodioxole were used to compare thedifferences amoning six fruiting bodies of A.cinnamomea from six woods(Cinnamomum camphor、Ci kanehirae 、Ci micranthora、Cryptomeria japonica、Cunninghamia lanceolata and Cu lanceolata var konishii). The HPLC chromatograms of six basidiomes from six woods were similar and the eight indicating components were presentedin the six basidiomes. During the five of forest investigation,75 speciemensof marcofungi were collected.The﹙30 isolates were cultured on pure medium including 3 isolates Microporus affinis、3 isolates of Rigidoporus microporus、2 isolates of Ganoderma australe、 3 isolates of G.lucidum、5 isolates of G.tropicum、6 isolates of Phellinus spp. and 9 unidentified isolates.