Gene expression analysis of NT193 cells with an epithelial (E) and mesenchymal (M) phenotype

Cells have been collected by limited dilution and mRNA from 3 consequtive passages of cells cultured in Dulbecco's modified Eagle's medium (DMEM Dutscher L0104-500) containing 4.5 g/L glucose, L-glutamine, sodium pyruvate supplemented with 10% of inactivated fetal bovine serum (FBS, Dutscher S1510-500), penicillin (10 000 U/mL), streptomycin (10 mg/mL) (Dutscher P06-07100) were used for RNA seq analysis.