Selective advantage of epigenetically disrupted cancer cells via phenotypic inertia [scRNA-seq]

Identification of gene expression changes at the single-cell level induced by glutamine deprivation in melanoma cells lacking various epigenetic regulators Overall design: scRNA-seq analysis of melanoma cells expressing 4-7sgRNAs targeting members of the PRC2 complex (EED, EZH2, SUZ12), histone H1B (HIST1H1B), the chromatin remodeller SMARCD1 and a non-expressed control gene (TNP2), and grown in either unperturbed conditions (d0) or under glutamine deprivation (d1, d2, d12). With the guild sequences (guides.csv) in combination with the sample pairings (indicated in column "characteristic: Gex-CRISPR"), the 10x library type information, the fastq files can be used to quantify the libraries using the cellranger count software from 10x Genomics.