CircRNA sequencing analysis of PTMC with lateral lymph node metastases and related clinical value studies of circRNA.

High-throughput sequencing was used to detect the expression profile of circRNA in PTMC of lymph node metastasis in lateral cervical region, and RT-qPCR was used to detect the expression of circRNA in cancer tissues and normal tissues adjacent to cancer. The receiver operating characteristic (ROC) curve and the area under ROC curve (AUC) were used to evaluate whether CircRNAs could be used as a biomarker for the diagnosis of thyroid cancer. Bioinformatics was used to predict the PTMC-related ceRNA mechanism of lateral cervical lymph node metastasis. 4 pairs of cancer tissues and normal paracancer tissues PTMC patients with cervical lymph node metastasis treated in the Department of Thyroid Surgery of Yuhuangding Hospital of Yantai with complete data were collected. The patients' cancer tissues and normal paracancer tissues were collected and stored in the refrigerator at -80℃ for long-term storage for high-throughput sequencing.