[Data Record 1]: qPCR validations

DESCRIPTION qPCR was used to assess the enrichment of ChIP and MeDIP experiments and to validate the integrity of sequencing libraries FILES Validation of ChIP-seq library enrichment: 20130426-CA1-1h-K27ac-K79me3-K4me3-H3-lib_qPCR.xlsx - validation of ChIP-seq libraries for samples from CA1 taken 1 hour after conditioning and immunoprecipitated for H3K27ac, H3K79me3, H3K4me3 or H3 20130715-ACCnaiveK76me3K27ac_qPCR.xlsx - validation of ChIP-seq libraries for naive samples from ACC and immunoprecipitated for H3K76me3 or H3K27ac 20131125-CA1-Naive_qPCR.xlsx - validation of ChIP-seq libraries for naive samples from CA1 20150122-CA1-K9ac_qPCR.xlsx - validation of ChIP-seq libraries for naive samples from CA1 and immunoprecipitated for H3k9ac Validation of MedIP-seq library enrichment: 20150729-MeDIP-LIB-ACC_qPCR.xlsx - MedIP-seq library validated for genes Cobl, Loxhd1, Vrk1 and GAPDHenh (-) 20150802-ACC-lib_qPCR.xlsx - MedIP-seq library validated for genes Nova1 and Reelin DATA GENERATION qPCR experiments were run on Roche LighCycler 480, and the machine was used to export the Ct values. The resulting output was imported into pyQPCR (version 0.10dev) using dilutions of inputs as standards, along with the following parameters: Calculation type: Absolute quantification Confidence interval: 90.00% Machine: Roche LighCycler 480 Maximum E(Ct): 0.30 Minimum Ct: 35.00