Transcriptional dynamics of hepatic stellate cells during first hours of in vitro activation

Transcriptional alterations during the first hours of hepatic stellate cell activation were determined using RNA Sequencing. Cultured hepatic stellate cells were collected for RNA Sequencing at several timepoints during the first 24 hours. Illumina NovaSeq SP was used for sequencing. Overall design: Bulk RNA sequencing of cultured mouse hepatic stellate cells after following timepoints: freshly isolated (n = 2) 3hours (n = 3) 6hours (n = 3) 12hours (n = 3) 18hours (n = 3) 24hours (n = 2) 96hours (n = 3)