Hispanic Lupus GWAS_HISPSLEI Study

The study comprises SLE patients recruited from specialist rheumatology clinics from the United States, Mexico, Argentina, Chile and Peru. Two strategies were used to enrich samples for Native American ancestry: a. previously genotyped Hispanic and Latin American and US Native American individuals were selected according to their ancestry based on 253 ancestry informative markers as part of the Lupus Large Association 2 Study. The approximate 35% of the samples were primarily of European and Native American admixture was selected; b. the recruitment of patients took place primarily in countries with little African admixture. All samples were genotyped on the HumanOmni1-Quad BeadChip using manifest H at the Oklahoma Medical Research Foundation. The Illumina clustering algorithm with GenomeStudio v2011.1 was used. ]]>
This study is a case-control GWAS comprised of 1393 SLE cases and 886 controls. All samples were genotyped on the HumanOmni1-Quad BeadChip using manifest H at the Oklahoma Medical Research Foundation. The Illumina clustering algorithm with GenomeStudio v2011.1 was used. SLE was the trait of interest (2=SLE Case, 1=Control), and we adjusted for two principal components. Inclusion: informed consent, fulfill ACR 1982 criteria for classification of SLE Controls matched by age and gender and site of recruitment ]]>
The sample collection has been ongoing since 1999 for the samples originating in Latin America and with the later joining of groups within the SLEGEN Consortium. With the formation of the SLEGEN Consortium, the group was able to obtain funding for a P01 (2010-2013) and an RC1 (2010-2011) to perform genome-wide association scan in patients with lupus of Hispanic (Project #3), African and Asian ancestry. Using data from the LLAS2 study, samples were selected with the aim of enriching for Native American ancestry.]]>