The polycomb protein MEL-18/PCGF2 regulates ErbB phosphorylation in HER2-positive breast cancer

Gene expression analysis on MEL-18-knockdown BT474 cells. MEL-18, a polycomb group protein and a member of the polycomb repressive complex 1 (PRC1), have suggested as a tumor suppressor in several cancer, including breast cancer. The results provides that the depletion of MEL-18 in HER2-positive breast cancer causes the activation of ErbB signaling pathway. We proposed that MEL-18 is a novel prognostic and therapeutic marker for HER2-positive breast cancer. Total RNA was isolated from the cells using Trizol reagent. BT474 cells stably infected with lentiviruses encoding MEL-18 shRNA (shMEL) compared to its control (shCon)