Genome-wide mapping of Ccp1 association sites in fission yeast. Schizosaccharomyces pombe

In our genetic screen, we found that Ccp1 is an epigenetic stability modulator in centromeres and heterochromatin in S. pombe. In interphase cells,Ccp1-GFP enriched at centromeres. To determine the association sites of Ccp1 with the centromeres and to characterize the global distribution pattern of Ccp1 throughout the genome, ChIP-seq were performed for Myc-tagged Ccp1 (C-terminal fusion protein). The result revealed that Ccp1 associates with the central core regions (cnt and imr) within every single centromere. Overall design: ChIP-seq (anti-Myc) were performed to wild-type (no tag) and ccp1-myc (C-terminal fusion) starins simultaneously.