Single cell RNA Sequencing analysis on CD45 positive cells isolated form Lewis Lung Carcinoma (LLC) tumors.
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE118931
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The goal of this study is to compare transcriptome profiling of different populations of CD45 immune cells of isolated from LLC tumors from miR-21 conditionally deleted on LysM expressing cells (mir-21 flox/flox; lysmCre) mice vs. control (mir-21flox/flox) mice. LLC cells were subqutaneously implated in 8 weeks old control (mir-21flox/flox) or miR-21 conditionally deleted on LysM expressing cells (mir-21 flox/flox; lysmCre) mice. After 14 days tumors were isolated and collagenase diggested then Cd45 positive cells were FACS sorted and RNA was straced for subsequent RNA-seq analysis.
创建时间:
2019-11-12



