Lower limits of detection of MVT and static qPCR.

1 NAE, flat-glass Nucleic Acid Extraction cards (BCSI); Qiagen, Qiagen AllPrep Total Nucleic Acid kit.2 TSB, trypticase soy broth; 7H9, Middlebrook 7H9 broth with ADC enrichment. All stimulations at 37°C except A. hydrophila, which was stimulated at 30°C.3 LOD, limit of detection. The LOD of MVT was the lowest density of spiked bacteria to exhibit a positive result, defined as ΔCt > 1 in three out of three replicates. MVT was always negative in the absence of spiked bacteria.4 LOD, limit of detection. The LOD of qPCR was the lowest density of spiked bacteria to exhibit a positive result, defined as a stronger signal (lower Ct value) than all three no-bacteria controls, in three out of three replicates.5 Experiment depicted graphically in Figure 1.