Supplementary Material for: Plasma Phospholipid Long-Chain n-3 Polyunsaturated Fatty Acids and Body Weight Change

<i>Objective:</i> We investigated the association between the proportion of long-chain n-3 polyunsaturated fatty acids (PUFA) in plasma phospholipids from blood samples drawn at enrollment and subsequent change in body weight. Sex, age, and BMI were considered as potential effect modifiers. <i>Method:</i> A total of 1,998 women and men participating in the European Prospective Investigation into Cancer and Nutrition (EPIC) were followed for a median of 4.9 years. The associations between the proportion of plasma phospholipid long-chain n-3 PUFA and change in weight were investigated using mixed-effect linear regression. <i>Results:</i> The proportion of long-chain n-3 PUFA was not associated with change in weight. Among all participants, the 1-year weight change was –0.7 g per 1% point higher long-chain n-3 PUFA level (95% confidence interval: –20.7 to 19.3). The results when stratified by sex, age, or BMI groups were not systematically different. <i>Conclusion:</i> The results of this study suggest that the proportion of long-chain n-3 PUFA in plasma phospholipids is not associated with subsequent change in body weight within the range of exposure in the general population.