Temperature-induced Unfolding Parameters for the hFXN Variants.
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The predicted ΔCP NU values asuming the complete unfolding of the molecules are 1.9 and 1.7 kcal mol−1 K−1 for hFXN90–210 and for hFXN90–195, respectively. [68] The parameters were calculated by nonlinear least square fit of the data shown in Figure 5B as described in Materials and Methods, using equations 1 and 2. ΔGNU20 C is the free energy of unfolding at 20°C and ΔGNUMAX is the highest value of free energy.
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2015-12-02



