Bryan Crawford (2011) CIL:12665, Danio rerio. CIL. Dataset

Transmission EM showing tight junctions apically and adherens junctions laterally between cells of the enveloping layer of a 24 hr (prim-5) Zebrafish embryo. Specimens fixed in Karnofsky's (overnight at 4˚C) followed by postfixing in 4% osmium tetroxide in .2M cacodylate for 1h. The resin was LX-112 and the stain was uranyl acetate and lead citrate. This image is part of a series taken by Bryan Crawford while he was at the University of Washington. They are part of the Zebrafish--The Living Laboratory CD made available by Mark Cooper and described in Methods in Cell Biology Volume 77, 2004, Pages 439-457.

传输电子显微镜图像展示了24小时（prim-5）斑马鱼胚胎外包层细胞顶端的紧密连接和侧面的粘附连接。标本经Karnofsky固定（过夜，4°C）后，在0.2M草酸镉溶液中用4%的四氧化锇进行后固定处理1小时。树脂为LX-112，染色剂为铀酰乙酸和铅柠檬酸盐。该图像为布莱恩·克劳福德在华盛顿大学期间所拍摄的一系列图像之一。这些图像收录于由马克·库珀提供的《斑马鱼——活体实验室》CD中，并在2004年出版的《细胞生物学方法》第77卷第439-457页中进行了描述。