OsterlundJBC_Figure S2

<b> Figure S2 data </b> <br><br>
Includes: <br>
-Panels A,C,D,E,F additional biological replicates acquired to supplement data shown in Figure 1D and Figure 2C,D,E,F respectively. Date acquired, Cell line, and mCerulean3-donor is given within each Excel worksheet. Raw binding curve data was binned by Venus intensity. Here we provide the resulting binned data, and fitting parameters exported from GraphPad prism used to create the binding curves that were displayed in the Supplementary.<br><br>
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AnnexinV (AnV) positivity, TMRE negativity, and/or nuclear area condensation ('SN' = 'small nuclear area') were used as a marker of cell death in panels G-H.
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-Panel G, cell death was measured in BMK, HCT116 or BMK/HCT116 cells with BAX and BAK knocked out ('DKO'). Here we provide the mean cell death measured in Venus-positive cells from 3 biological replicates. <br><br>
-In Panel H, cell death was measured in HEK293T cells and from one technical replicate, we binned data by Venus intensity as a measure of protein concentration to examine trends.