Integration of erm(B)-containing elements through large chromosome fragment exchange in Clostridium difficile

In Clostridium difficile, erm(B) genes are located on mobile elements like Tn5398 and Tn6125. In previous studies, some of these elements were transferred by conjugation-like mechanisms, mobilized in trans by helper conjugative systems. In this study, we analyzed the genomes of several recipient strains that acquired either Tn5398 or Tn6125-like elements. We demonstrated that the integration of the transposons in the genome of the recipient cell was always due to homologous recombination events, involving exchange of large chromosomal segments.