RNA sequencing in kidneys of MUC1 Kidney Disease mouse model with and without treatment

MUC1 kidney disease mouse model generated by knock-in of the human mutated MUC1-fs gene. The mice were treated for 7 days, daily with either vehicle control or BRD4780, a compound showed to be beneficial to disease cells in-vitro. The kidneys were then removed and the bulk RNA was sequenced. The goal of this study was to undersand the effect of the compound on the diseased kidneys and to gain insights to its mechanism of action. Overall design: MUC1 kidney disease mouse models in quatriplate were treated with either vehicle or BRD4780 daily for 7 days, followed by kidney removal, RNA isolation and bulk RNA sequencing