Metabolome data from bovine preovulatory follicular fluid

The intrafollicular milieu influences mammalian fertility by providing the microenvironment for oocyte growth and maturation. A number of studies have linked abundance of follicular fluid metabolites to oocyte developmental competence and pregnancy outcome. Few studies have interrogated the preovulatory follicular fluid metabolome in cattle. This dataset includes preovulatory follicular fluid metabolome profiles from non-lactating Jersey cows. Ultra-High-Performance Liquid Chromatography-High Resolution Mass Spectrometry was performed on preovulatory follicular fluid samples and Xcalibur (RAW) files were converted to an open-source mzML format (msconvert software; ProteoWizard package). The converted files were processed using the Metabolomic Analysis and Visualization Engine (MAVEN; mzroll software, Princeton University) to complete an untargeted analysis of the liquid chromatography mass spectrometry data. The pre-processed peak data tables generated by MAVEN are provided in this data..., Ultra-High-Performance Liquid Chromatography - High Resolution Mass Spectrometry (UHPLC-HRMS) was performed on follicular fluid samples at the Biological and Small Molecule Mass Spectrometry Core (RRID: SCR_021368) at the University of Tennessee, Knoxville. Sixty μL aliquots of follicular fluid samples (n=29) were thawed at room temperature, and metabolites within each sample were extracted using a 40:40:20 methanol/acetonitrile/water solution with 0.1 M formic acid following a previously reported procedure. An extraction solvent was added to the samples which underwent agitation and vortexing before samples were then chilled at -20°C for 20 min. Once samples were properly chilled, the tubes were centrifuged to form pellets and remove debris from the sample. A second set of 2 mL microcentrifuge tubes were used to collect the supernatant from the first set of tubes. The extraction solvent was again dispensed into the first set of tubes, which contained the pellet, and the mixture was pip..., Microsoft Excel is required to open the dataset.Â