Nucleocytoplasmic transport is highly selective, efficient, and is regulated by a poorly understood mechanism involving hundreds of disordered FG nucleoporin proteins (FG nups) lining the inside wall
We performed gene expression analyses using total RNA-seq and TRAP-seq Overall design: GFP-RPL10A TRAP-seq under control vs. LSM12-depleted SH-SY5Y cells
Cells were maintained in complete media (CM) or starved for 24 hours in HBSS prior to imaging. The p:s ratios and representative images are presented for: Nup58-mEGFP(-8).
How microorganisms resist nutrient-deficiency is important for understanding the pervasive prosperity of microbes in severe nutrient conditions. In laboratory culture, E. coli can survive for a long p